Why was it important to include a positive control and a negative...

Question

Why was it important to include a positive control and a negative control in the PCR analysis?

Accepted Answer

Hi everyone. Here's our next question. Why is tak preliminaries being used in pcR, what's your call? We use pcR to amplify a specific um segment of D. N. A. And it has three steps. The first being DNA duration. So we separate the DNA strands and that's done by healing To 92°C. Then our second step is primary binding And our 3rd step Primer extension. However, let's zoom in on this DNA duration step. There aren't many proteins that can withstand a temperature of 92°C. and DNA polymerase is an example of that. So we need to use a version of polymerase that can withstand this high heat and that is tack pollen race. So tactile embrace has high heat the ability to withstand this temperature. So that's why we need to use it in PCR. So when we look at our answer choices, we see choice A. Is it is thermo stable and that would be a correct answer. Well, just briefly look over our other answer choices. Choice B says it is not primary dependent. Well that is not correct because you do need primers for it to start from. I see says it can carry out reverse transcription. No that's not what we use it for. We use it to synthesize DNA not to synthesize RNA from D. N. A. And Choice D. Says it can start memorization by itself. That's also not correct. It starts with the addition of the primers and then it extends the sequence from the primers. So our answer to why we use tactile embrace is choice A. It is thermo stable, see you in the next video.

Why was it important to include a positive control and a negative control in the PCR analysis?

Watch next