Potato blight causes potato plants to shrivel and rot. The diseas...

Question

Potato blight causes potato plants to shrivel and rot. The disease is caused by the pathogen Phytophthora infestans, infamous for its role in Ireland's Great Potato Famine in the mid-1840s. The disease can devastate crops during wet weather, sometimes leading to total crop loss. Researchers aim to use recombinant DNA methods to transfer blight resistance genes from resistant varieties into susceptible varieties of potato.

Explain how restriction endonucleases and DNA ligase could be used to insert a potato blight resistance gene into a plasmid.

Accepted Answer

Hello everyone. And in today's video we have the following problem which one of the following is correctly match with his role in recombinant DNA technology. I want you to recall from previous videos what this recombinant DNA technology is and it involves cutting up or gene of interest and then sealing it within plasmids as we see here so that we can then insert it into another organism so that it can express it. It is a form of D. N. A editing that is used in the modern world. So now that we know this, let's go over all of these so that we can solve the problem beginning by answer choice. A reverse transcriptase will reverse transcriptase is an enzyme that is able to turn RNA into DNA. Because of this the function assigned to it cutting up the gene of interest is not going to be the correct one. And we're going to cancel it out. Then we have restriction and the nucleus is formation of C. D. N. A. Well restriction in the nucleus is the function is actually the cutting up of the gene of interest. Because of this formation of C. D. N. A. Is not going to be the correct answer choice. And we're going to cancel this out as well. Then we have vector expression of the gene of interest vector is something that is used to carry a certain gene of interest for example into an organism. So that plus meat for example is a vector. The expression of the gene of interest is not really what it does. It is a carrier of the gene of interest into the organism. So we're going to cancel it out as well. And this all leaves us with DNA like and sealing the gene of interest into the plasma. So it is what allows of interest to be inserted into the plasmid. And this is actually what this enzyme DNA. Like those. And what DNA like does is that it fixes breaks in the DNA. So it is going to find these DNA of interest broken apart, and it is going to fix it again into the plasmid so that it can be introduced into the organism of interest. So we're going to highlight answer choice e as the correct answer. Thank you very much for sticking around until the end of this video. And I hope this helped you.

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