Question

What are the advantages of using a restriction enzyme whose recognition site is relatively rare? When would you use such enzymes?

Accepted Answer

Hello, everyone and welcome to today's video. So a rare color enzyme is a restriction enzyme with the recognition sequence that occurs rarely in the genome. Which of the following statements is true regarding these rare color enzymes. So before we jump into the answer traces, please notice that we have two restriction enzyme that we're comparing. We have not one and H A E three. So let's just go over some of the facts regarding these two restriction enzymes. First of all, not one is going to have these eight based along recognition sites and it is going to be used to generate larger DNA fragments while H A E three is going to have these four based per long recognition site and is used to generate smaller DNA fragments. So keep that in mind as we go over each of the answer choices. So first of all, we're going to look at answer choice B we have that H E three is a rare color enzyme without four VP recognition site. This is correct generating larger DNA fragments. Well, remember that is used to generate smaller nor larger DNA fragments were going to cancel this out. Then we have not. One is a rural culture and sign without four based per long recognition site, which is going to be incorrect as we see here, generating smaller DNA fragments, which is also going to be incorrect. As we see here, this is a completely incorrect statement. So we're going to cancel it out. Then we have the age A E three is a rare cutter enzyme with that eight based prolonged recognition site. As we see here, this is going to be incorrect, generating larger DNA fragments, which is also going to be incorrect because of this. We're also going to cancel this out. Then we have not, one is a rare color enzyme with an eight based for long recognition site, which is correct, generating larger DNA fragments, which is also correct because of this answer choice. A is the only correct answer choice. And we're going to highlight it as a final answer to our question. I really hope this helped you and I hope to see you on the next one.

What are the advantages of using a restriction enzyme whose recognition site is relatively rare? When would you use such enzymes?

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