Question

What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.

Accepted Answer

Welcome back everyone. Let's look at our next question. Which of the following libraries only contains genes that were expressed in cells at the time. The library was created. So we look at our answer choices. Um We have choices of genomic libraries, complementary DNA libraries, Genomic DNA libraries or both A. And C. Well if we want to think about a library that only shows expressed genes well expressed genes are going to be genes that have gotten transcribed into M. R. N. A. So what we want to look at is choice be complementary DNA libraries. These complementary D. N. A. Are small fragments of D. N. A that are complementary two M RNA molecules. So that means that those DNA fragments represent only the genes that are expressed. So because they are come from M RNA transcripts. So when we look at our other answer choices. Choice A genomic libraries. Well this would be small DNA fragments representing all of the D. N. A. In the genome. So including coding and non coding DNA and genes that have that aren't being expressed. So that's not our correct answer. Therefore, Choice D. Both A. N. C. Is not our answer. Choice C. Genomic DNA library is just another name for a genomic library. So choice C. Is not our answer. So again, which the following libraries only contains genes that were expressed in cells at the time library was created and this choice be complementary DNA libraries. See you in the next video

What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.

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