Question

List the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs and YACs provide over plasmids as cloning vectors?

Accepted Answer

Hello everyone and welcome to today's video. So these cloning vectors, our genomes that can accept the target DNA and increase the number of copies through its autonomous replication. Which of the following statements is false. Remember that we're looking for the incorrect regarding these bacterial artificial chromosomes and genes, artificial chromosome vectors. Well, let's go over each of the answer choices so that we may solve the problem. So these G's artificial chromosome vectors are produced based on specific regions of the chromosome while this is correct. Whereas these bacterial artificial chromosome vectors are produced based on the F plasmid which is going to be present in bacteria. Both statements are going to be true. So we're going to cancel this out because we're looking for the false one, the incorrect one and modern answer choices. Then we have these GS artificial chromosome vectors have high cloning capacity while these bacterial artificial chromosome vectors have low cloning capacity. Remember that geese are going to be, you carry it and they're going to have a much larger genome than these bacterial. So they can accept larger inserts, which is going to amplify their capacity for cloning because of this, this is a correct statement. So we're going to cancel it out. Then we have seen these artificial chromosome vectors are going to be circular or bacterial. Artificial chromosome vectors are going to be linear. Well, remember that this just artificial chromosomes are going to be coming from fungi and fungi have linear chromosomes. On the other hand, these bacterial artificial chromosomes are going to be coming from precarious from bacteria and they are going to have circular chromosomes because of this. These are inverted. They shouldn't be like this. This is incorrect. So we're going to highlight answer choice c as a correct answer to our question. I really hope this helped you and I hope to see you on the next one.

List the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs and YACs provide over plasmids as cloning vectors?

Watch next