Question

What roles do restriction enzymes, vectors, and host cells play in recombinant DNA studies? What role does DNA ligase perform in a DNA cloning experiment? How does the action of DNA ligase differ from the function of restriction enzymes?

Accepted Answer

Hi, everybody. Let's take a look at this practice problem together. Blank is the molecule glue that is used in the cloning experiment to join two fragments of D N A to seal the gaps between them. A quick glance at the answer options. And we can see they all end in A S E. That means our molecule glue is an enzyme. Our answer will be an enzyme And it's an enzyme that Catalyze is a bond and joins two fragments of DNA. So let's discuss our answer options. We've got a restriction endo nuclear's now recall that a restriction endo nuclear's recognizes certain DNA sequences called restriction sites. And that cleaves at these sites. It's common for them to be used in recombinant DNA technology. And therefore, this is the opposite of what we are looking for. So A is not the correct answer. Then we've got be topo Y summaries. Recall that topo I summaries breaks and rejoins D N A to fix super coiling. Now super coiling can occur as a result of DNA replication and transcription. Therefore, since this breaks and rejoins D N A option B is not the correct answer. Then we have options. See DNA, like gaze and the prefix liga means to bind. So this is an enzyme that binds DNA fragments together. It capitalizes the formation of a phosphor diaspora bond and allows the nucleotides to be connected to each other. Therefore, c is the correct answer. Alright, everyone, I hope you found this helpful and I'll see you soon for the next practice problem.

What roles do restriction enzymes, vectors, and host cells play in recombinant DNA studies? What role does DNA ligase perform in a DNA cloning experiment? How does the action of DNA ligase differ from the function of restriction enzymes?

Watch next