Question

In the context of recombinant DNA technology, of what use is a probe?

Accepted Answer

Hi, everyone. Welcome back. Let's look at our next question. A probe is a single stranded DNA or RNA sequence used to search for its complementary sequence in a sample genome, which of the following is exactly complementary to the gene of interest. And our answer choices are a hetero lacasse probe. B ala go nucleotide probe, see homologous probe or D all of the above. So we have the potential for more than one correct answer. Well, here we've got our prefix is giving us a hint here. We've got a choice, a hetero ologists about hetero prefix and see homologous that homo prefix different and same. So if we're looking for the probe, that would be exactly complimentary the team of interest. If we don't remember off the top of our heads, hopefully that prefix of homo or same would point us to choice C homologous probe. And that is indeed our answers. That's when you have a complementary DNA probe that is exactly complementary to the gene that you're looking for. Choice. A hetero lacasse probe is when it's not quite the same, it doesn't match exactly. Um And an example might be when you might use a DNA sequence from a different species to try and hunt for a gene in that gene of interest in a different species. So you may be looking for one that will be not quite exactly the same might be a few base pairs off. But that's why choice A isn't correct. Choice. B is Olivo nucleotide probe. That's just the generic term for a probe made of a short sequence of DNA that could be hetero lacasse or homologous. So that's why that's not a correct answer. And then of course, d all of the above is incorrect since we do have some incorrect answers here. So again, um a probe is a single strand of DNA or RNA sequence used to search for its complementary sequence, which of the following is exactly complementary to the dreaming of interest. And that's choice C homologous probe. See you in the next video.

In the context of recombinant DNA technology, of what use is a probe?

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