The two gels illustrated contain dideoxynucleotide DNA-sequencing...

Question

The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and mutant segment of DNA corresponding to the N-terminal end of a protein. The start codon and the next five codons are sequenced.

Identify the template and nontemplate strands of DNA.

Accepted Answer

Hi, everyone. Welcome back. Let's look at our next problem. It says select the accurate statement about the roles played by the leading and lagging strands during DNA replication choice. A the template strand contains the mutant segment of DNA and the non template strand contains the wild type segment choice B, the template strand contains the wild type segment of DNA. And the non template strand contains the mutant segment choice C, the template strand contains the start codon and the next five codons and the non template strand is complementary to the template strand or choice D the template and non template strands cannot be identified from the given data. Well, this is kind of a confusing question to look at because our problem that's given to us is asking us about the roles played by the leading and lagging strands during DNA replication. But our answer choices then go on to talk about template and non template strands. Well, template and non template strands refer to the roles played by the DNA strands in transcription, not in replication. So leading and lagging strands refer to as DNA is being replicated. As her question says, the double helix and wins. And the two strands of DNA are copied into two new strands of DNA. And it has to do with the direction and the fact that DNA polymerase can only synthesize in the five prime to three prime direction. So you've got your leading strand that's going along continuously, five prime to three prime and your lagging strand that has to work in fragments. But all of our answer choices are then asking us to identify which is the template or non template strand. The template strand being the one that is being used as a template obviously for the synthesis of the R N A strand. Well, we have no idea which strand leading or lagging when the two are being copied goes on to become the template strand for R N A synthesis. There's no link between these two things. So our answer here is going to be choice D, the template and non template strands cannot be identified from the given data because we're talking about two different processes. So choice D is the answer we're looking for here. See you in the next video.

The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and mutant segment of DNA corresponding to the N-terminal end of a protein. The start codon and the next five codons are sequenced. Identify the template and nontemplate strands of DNA.

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