Question

Although many cloning applications involve introducing recombinant DNA into bacterial host cells, many other cell types are also used as hosts for recombinant DNA. Why?

Accepted Answer

Hello everyone and welcome to today's video. So direct gene transfer is a method of introducing recombinant D N A into host cells, which of the following methods is not an example of direction transfer. And remember that interactions transfer, we only have the medium and the cell. There is nothing in between here that use directly transferring the D N A into the cell. So let's go over each of the answer. Choices. We have micro injection or micro injection uses a micro needle to transfer DNA directly into the nucleus by puncturing the plasma membrane. This is an example of direct genes transfer. So we're going to cancel it out, then we have been gone. And this is very interesting because it is going to be shooting these gold particles coated with DNA. And these are going to enter the cell being also a direct method of gene transfer. So we're going to cancel this. Remember that we're looking for the example that is not a a method of directions transfer. So keep that in mind, then we have electric operations. So we have cells in a medium, there's very high volts of electricity in the medium. So this is going to affect the plasma membrane allowing DNA to enter directly into the cell. We're also going to cancel it out because this is also a form of direct transfer and this is all leaving us with answer choice C or virus. So here we have, for example, a bacteria of each, this virus is going to be used to transfer the DNA into the cell. However, we're putting it inside the virus. So then then the virus can put it inside of the cell. So here we have a vector, we have a method that is not directly transferring the D N A from the medium into the cell. We have an intermediate because of this virus or the bacteria phages, which is answer choice C is going to be there. Correct answer to our question. I really hope this helped you and I hope to see you on the next one.

Although many cloning applications involve introducing recombinant DNA into bacterial host cells, many other cell types are also used as hosts for recombinant DNA. Why?

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