In a dideoxy DNA sequencing experiment, four separate reactions are carried out to provide the replicated material for DNA sequencing gels. Reaction products are usually run in gel lanes labeled A, T, C, and G.
Why is incorporation of a dideoxynucleotide during DNA sequencing identified as a 'replication-terminating' event?
Play a video:
This video solution was recommended by our tutors as helpful for the problem above.
Was this helpful?
Master Methods for Analyzing DNA and RNA with a bite sized video explanation from Kylia Goodner