Alkaptonuria is a human autosomal recessive disorder caused by mu...

Question

Alkaptonuria is a human autosomal recessive disorder caused by mutation of the HAO gene that encodes the enzyme homogentisic acid oxidase. A map of the HAO gene region reveals four BamHI restriction sites (B1 to B4) in the wild-type allele and three BamHI restriction sites in the mutant allele. BamHI utilizes the restriction sequence 5′-GGATCC-3′. The BamHI restriction sequence identified as B3 is altered to 5′-GGAACC-3′ in the mutant allele. The mutation results in a Ser-to-Thr missense mutation. Restriction maps of the two alleles are shown below, and the binding sites of two molecular probes (probe A and probe B) are identified.

DNA samples taken from a mother (M), father (F), and two children (C1 and C2) are analyzed by Southern blotting of BamHI-digested DNA. The gel electrophoresis results are illustrated.

In a separate figure, draw the gel electrophoresis band patterns for all the genotypes that could be found in children of this couple.

Accepted Answer

Welcome back everyone. Here's our next question. Gel electrophoresis is a method for the separation and analysis of proteins. DNA and other macro molecules based on their size and charge. Which of the following factors does not affect the macromolecule speed of movement. A, the type of Arose Gel used B the voltage applied to the gel C the concentration of buffer solution or D the color of the macro molecules. So we're thinking about how macro molecules move through a gel in this technique of gel electrophoresis. So let's look at our answer choices and evaluate them. Remembering that we're looking for which one does not affect the speed with which these macro molecules move through the gel. So choice A is the type of Agarose gel. Well, this does affect their speed of movement because the macro molecules move through the pores of the gel. So the type of gel used will affect the size of the pores and therefore the speed at which those molecules move through the gel. So choice A is not our answer. Then choice B says the voltage applies to the gel. Well, this will affect the speed, the higher the voltage the higher the speed with which these charged molecules move. So that's not our answer. Choice either. Then do I see the concentration of the buffer solution? Well, the concentration, the so the amount of ions in the buffer affect the conductivity of the gel and therefore the speed with which the molecules move. So choice c also not our answer. So finally, we've just got choice D left the color of macro molecules and color is not going to affect the speed with which these molecules move. So choice D is our answer. The color of the macro molecules will not affect the macromolecule speed of movement. See you in the next video.

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