Laboratory Experiments in Microbiology, 12th edition

  • Ted R. Johnson, 
  • Christine L. Case

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With Laboratory Experiments in Microbiology, you'll learn basic microbiology techniques and applications through 57 class-tested exercises. This manual covers every area of microbiology for undergraduate study across diverse disciplines and is designed to help you develop essential laboratory thinking skills.

Published by Pearson (July 14th 2021) - Copyright © 2019

ISBN-13: 9780137546503

Subject: Microbiology

Category: Introductory Microbiology

Table of contents

Part I: Microscopy
1. Use and Care of the Microscope
2. Examination of Living Microorganisms

Part II: Handling Bacteria
3. Microbes in the Environment
4. Transfer of Bacteria: Aseptic Technique

Part III: Staining Methods
5. Preparation of Smears and Simple Staining
6. Negative Staining
7. Gram Staining
8. Acid-Fast Staining
9. Structural Stains (Endospore, Capsule, and Flagella)
10. Morphological Unknown

Part IV: Cultivation of Bacteria
11. Isolation of Bacteria by Dilution Techniques
12. Special Media for Isolating Bacteria

Part V: Microbial Metabolism
13. Carbohydrate Catabolism
14. Fermentation
15. Protein Catabolism, Part 1
16. Protein Catabolism, Part 2
17. Respiration
18. Unknown Identification and Bergey's Manual

Part VI: Microbial Growth
19. Oxygen and the Growth of Bacteria
20. Determination of a Bacterial Growth Curve: The Role of Temperature
21. Biofilms

Part VII: Control of Microbial Growth
22. Physical Methods of Control: Heat
23. Physical Methods of Control: Ultraviolet Radiation
24. Chemical Methods of Control: Disinfectants and Antiseptics
25. Chemical Methods of Control: Antimicrobial Drugs
26. Effectiveness of Hand Scrubbing

Part IIX: Microbial Genetics
27. Regulation of Gene Expression
28. Isolation of Bacterial Mutants
29. Transformation of Bacteria
30. DNA Fingerprinting
31. Genetic Engineering
32. Ames Test for Detecting Possible Chemical Carcinogens

Part IX: The Microbial World
33. Fungi: Yeasts and Molds
34. Phototrophs: Algae and Cyanobacteria
35. Protozoa
36. Parasitic Helminths

Part X: Viruses
37. Isolation and Titration of Bacteriophages
38. Plant Viruses

Part XI: Interaction of Microbe and Host
39. Epidemiology
40. Koch's Postulate

Part XII: Immunology
41. Innate Immunity
42. Agglutination Reactions: Slide Agglutination
43. Agglutination Reactions: Microtiter Agglutination
44. ELISA Technique

Part XIII: Microrganisms and Disease
45. Bacteria of the Skin
46. Bacteria of the Respiratory Tract
47. Bacteria of the Mouth
48. Bacteria of the Gastrointestinal Tract
49. Bacteria of the Urogenital Trac
50. Identification of an Unknown from a Clinical Sample
51. Rapid Identification Methods

Part XIV: Microbiology and the Environment
52. Microbes in Water: Multiple-Tube Technique
53. Microbes in Water: Membrane Filter Technique
54. Microbes in Food: Contamination
55. Microbes Used in the Production of Foods
56. Microbes in Soil: The Nitrogen and Sulfur Cycles
57. Microbes in Soil: Bioremediaton

A. Pipetting
B. Dilution Techniques and Calculations
C. Use of the Spectrophotometer
D. Graphing
E. Use of the Dissecting Microscope
F. Use of the Membrane Filter
G. Electrophoresis
H. Keys to Bacteria

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