Microbiology: A Laboratory Manual

Microbiology: A Laboratory Manual, 12th edition

  • James G. Cappuccino, 
  • Chad T. Welsh

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Overview

Microbiology provides you with a solid underpinning of microbiology laboratory work while putting increased focus on clinical applications and helping you build critical-thinking skills. The text includes clinical case studies, equipment and material checklists, experiments, governing body guidelines, and more.

Published by Pearson (June 14th 2021) - Copyright © 2020

ISBN-13: 9780137546527

Subject: Microbiology

Category: Introductory Microbiology

Table of contents

Part I: Basic Laboratory Techniques for Isolation, Cultivation, and Cultural
Experiment 1: Effectiveness of Hand Washing
Experiment 2: Culture Transfer Techniques
Experiment 3: Techniques for Isolation of Pure Cultures
Experiment 4: Cultural Characteristics of Microorganisms

Part II: Microscopy
Experiment 5: Microscopic Examination of Stained Cell Preparations
Experiment 6:Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount

Part III: Bacterial Staining
Experiment 7: Preparation of Bacterial Smears
Experiment 8: Simple Staining
Experiment 9: Negative Staining
Experiment 10:Gram Stain
Experiment 11: Acid-Fast Stain
Experiment 12: Differential Staining for Visualization of Bacterial Cell Structures

Part IV: Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations
Experiment 13:Nutritional Requirements: Media for the Routine Cultivation of Bacteria
Experiment 14: Use of Differential, Selective, and Enriched Media
Experiment 15: Physical Factors: Temperature
Experiment 16: Physical Factors: pH of the Extracellular Environment
Experiment 17: Physical Factors: Atmospheric Oxygen Requirements
Experiment 18: Techniques for the Cultivation of Anaerobic Microorganisms
Experiment 19: Serial Dilution-Agar Plate Procedure to Quantitate Viable Cells
Experiment 20: The Bacterial Growth Curve

Part V: Biochemical Activities of Microorganisms
Experiment 21: Extracellular Enzymatic Activities of Microorganisms
Experiment 22: Carbohydrate Fermentation
Experiment 23: Triple Sugar-Iron Agar Test
Experiment 24: IMViC Test
Experiment 25: Hydrogen Sulfide Test
Experiment 26: Urease Test
Experiment 27: Litmus-Milk Reactions
Experiment 28: Nitrate Reduction Test
Experiment 29: Catalase Test
Experiment 30: Oxidase Test
Experiment 31: Utilization of Amino Acids
Experiment 32: Genus Identification of Unknown Bacterial Cultures

Part VI: The Protozoa
Experiment 33: Free-Living Protozoa
Experiment 34: Parasitic Protozoa

Part VII: The Fungi
Experiment 35: Cultivation and Morphology of Molds
Experiment 36: Isolation of a Soil Fungal Species
Experiment 37:Morphology, Cultural Characteristics and Reproduction

Part IIX: The Viruses
Experiment 38: Cultivation and Enumeration of Bacteriophages
Experiment 39: Isolation of Coliphages from Raw Sewage
Experiment 40: Propagation of Isolated Bacteriophage Cultures

Part IX: Physical and Chemical Agents for the Control of Microbial Growth
Experiment 41: Physical Agents of Control: Moist Heat
Experiment 42: Chemical Agents of Control: Chemotherapeutic Agents
Experiment 43: Determination of Penicillin Activity in the Presence and Absence of Penicillinase
Experiment 44: Chemical Agents of Control: Disinfectants and Antiseptics

Part X: Microbiology of Food
Experiment 45: Microbiological Analysis of Food Products: Bacterial Count
Experiment 46: Isolation of Salmonella from Raw Meat
Experiment 47: Microbial Fermentation

PART XI: Microbiology of Water
Experiment 48: Standard Qualitative Analysis of Water
Experiment 49: Quantitative Analysis of Water: Membrane Filter Method

PART XII: Microbiology of Soil
Experiment 50: Microbial Populations in Soil: Enumeration
Experiment 51: Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates
Experiment 52: Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique

PART XIII: Bacterial Genetics
Experiment 53: Enzyme Induction
Experiment 54: Bacterial Conjugation
Experiment 55: Isolation of a Streptomycin-Resistant Mutant
Experiment 56: The Ames Test: A Bacterial Test System for Chemical Carcinogenicity
Experiment 57: Utilization of Bacterial Plasmids
Experiment 58: Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA

PART XIV: Medical Microbiology
Experiment 59: Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries
Experiment 60: Normal Microbial Flora of the Throat and Skin
Experiment 61: Identification of Human Staphylococcal Pathogens
Experiment 62: Identification of Human Streptococcal Pathogens
Experiment 63: Identification of Streptococcus pneumoniae
Experiment 64: Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems
Experiment 65: Isolation and Presumptive Identification of Campylobacter
Experiment 66: Microbiological Analysis of Urine Specimens
Experiment 67: Microbiological Analysis of Blood Specimens
Experiment 68: Species Identification of Unknown Bacterial Cultures

PART XV: Immunology
Experiment 69: Precipitin Reaction: The Ring Test
Experiment 70: Agglutination Reaction: The Febrile Antibody Test
Experiment 71: Enzyme-Linked Immunosorbent Assay
Experiment 72: Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures

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