Microbiology: A Laboratory Manual, 12th edition

  • James G. Cappuccino, 
  • Chad T. Welsh

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Microbiology provides you with a solid underpinning of microbiology laboratory work while putting increased focus on clinical applications and helping you build critical-thinking skills. The text includes clinical case studies, equipment and material checklists, experiments, governing body guidelines, and more.

Published by Pearson (June 14th 2021) - Copyright © 2020

ISBN-13: 9780137546527

Subject: Microbiology

Category: Intro Microbiology

Table of contents

Brief Contents

Part 1 Basic Laboratory Techniques for Isolation, Cultivation, and Cultural

  • Experiment 1: Effectiveness of Hand Washing
  • Experiment 2: Culture Transfer Techniques
  • Experiment 3: Techniques for Isolation of Pure Cultures
  • Experiment 4: Cultural Characteristics of Microorganisms

Part 2 Microscopy

  • Experiment 5: Microscopic Examination of Stained Cell Preparations
  • Experiment 6:Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount

Part 3 Bacterial Staining

  • Experiment 7: Preparation of Bacterial Smears
  • Experiment 8: Simple Staining
  • Experiment 9: Negative Staining
  • Experiment 10:Gram Stain
  • Experiment 11: Acid-Fast Stain
  • Experiment 12: Differential Staining for Visualization of Bacterial Cell Structures

Part 4 Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations

  • Experiment 13:Nutritional Requirements: Media for the Routine Cultivation of Bacteria
  • Experiment 14: Use of Differential, Selective, and Enriched Media
  • Experiment 15: Physical Factors: Temperature
  • Experiment 16: Physical Factors: pH of the Extracellular Environment
  • Experiment 17: Physical Factors: Atmospheric Oxygen Requirements
  • Experiment 18: Techniques for the Cultivation of Anaerobic Microorganisms
  • Experiment 19: Serial Dilution–Agar Plate Procedure to Quantitate Viable Cells
  • Experiment 20: The Bacterial Growth Curve

Part 5 Biochemical Activities of Microorganisms

  • Experiment 21: Extracellular Enzymatic Activities of Microorganisms
  • Experiment 22: Carbohydrate Fermentation
  • Experiment 23: Triple Sugar–Iron Agar Test
  • Experiment 24: IMViC Test
  • Experiment 25: Hydrogen Sulfide Test
  • Experiment 26: Urease Test
  • Experiment 27: Litmus–Milk Reactions
  • Experiment 28: Nitrate Reduction Test
  • Experiment 29: Catalase Test
  • Experiment 30: Oxidase Test
  • Experiment 31: Utilization of Amino Acids
  • Experiment 32: Genus Identification of Unknown Bacterial Cultures

Part 6 The Protozoa

  • Experiment 33: Free-Living Protozoa
  • Experiment 34: Parasitic Protozoa

Part 7 The Fungi

  • Experiment 35: Cultivation and Morphology of Molds
  • Experiment 36: Isolation of a Soil Fungal Species
  • Experiment 37:Morphology, Cultural Characteristics and Reproduction

Part 8 The Viruses

  • Experiment 38: Cultivation and Enumeration of Bacteriophages
  • Experiment 39: Isolation of Coliphages from Raw Sewage
  • Experiment 40: Propagation of Isolated Bacteriophage Cultures

Part 9 Physical and Chemical Agents for the Control of Microbial Growth

  • Experiment 41: Physical Agents of Control: Moist Heat
  • Experiment 42: Chemical Agents of Control: Chemotherapeutic Agents
  • Experiment 43: Determination of Penicillin Activity in the Presence and Absence of Penicillinase
  • Experiment 44: Chemical Agents of Control: Disinfectants and Antiseptics

Part 10 Microbiology of Food

  • Experiment 45: Microbiological Analysis of Food Products: Bacterial Count
  • Experiment 46: Isolation of Salmonella from Raw Meat
  • Experiment 47: Microbial Fermentation

PART 11 Microbiology of Water

  • Experiment 48: Standard Qualitative Analysis of Water
  • Experiment 49: Quantitative Analysis of Water: Membrane Filter Method

PART 12 Microbiology of Soil

  • Experiment 50: Microbial Populations in Soil: Enumeration
  • Experiment 51: Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates
  • Experiment 52: Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique

PART 13 Bacterial Genetics

  • Experiment 53: Enzyme Induction
  • Experiment 54: Bacterial Conjugation
  • Experiment 55: Isolation of a Streptomycin-Resistant Mutant
  • Experiment 56: The Ames Test: A Bacterial Test System for Chemical Carcinogenicity
  • Experiment 57: Utilization of Bacterial Plasmids
  • Experiment 58: Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA

PART 14 Medical Microbiology

  • Experiment 59: Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries
  • Experiment 60: Normal Microbial Flora of the Throat and Skin
  • Experiment 61: Identification of Human Staphylococcal Pathogens
  • Experiment 62: Identification of Human Streptococcal Pathogens
  • Experiment 63: Identification of Streptococcus pneumoniae
  • Experiment 64: Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems
  • Experiment 65: Isolation and Presumptive Identification of Campylobacter
  • Experiment 66: Microbiological Analysis of Urine Specimens
  • Experiment 67: Microbiological Analysis of Blood Specimens
  • Experiment 68: Species Identification of Unknown Bacterial Cultures

PART 15 Immunology

  • Experiment 69: Precipitin Reaction: The Ring Test
  • Experiment 70: Agglutination Reaction: The Febrile Antibody Test
  • Experiment 71: Enzyme-Linked Immunosorbent Assay
  • Experiment 72: Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures

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