Techniques for Microbiology: A Student Handbook, 1st edition

  • John M. Lammert

Techniques for Microbiology: A Student Handbook

ISBN-13:  9780132240116

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Overview

This vivid, full-color laboratory techniques handbook is an instructive, concise, graphical presentation of the skills and techniques required in an introductory microbiology lab. Clear visual instructions enable readers to carry out fundamental manipulations and procedures effectively and safely. KEY TOPICS: Demonstrates those techniques that will be used frequently for studying microbes in the laboratory. Has a safety section and frequent safety cautions throughout. Has a convenient, portable 6” x 9” trim size, a spiral binding and soft cover, making it ideal for use on the lab bench surface. It is priced inexpensively so that it will be suitable as a supplement to an in-house or commercial manual. MARKET: Companion to any introductory laboratory whether for biology majors or allied health majors.

Table of contents

Unit I — Safety first!

  

Safety in the microbiology laboratory

 

  Universal precautions

 

Unit II — Culturing bacteria and aseptic techniques

 

  Preparing culture media

 

  Sterilization

    The autoclave

    Dry heat

 

  Aseptic transfer of bacteria

    Adjusting the gas burner

    Preparing bacterial cultures in broth tubes and on agar slants

    Transfer colony from plate to broth and to agar slant

 

  Isolation of bacteria

    Preparing a pour plate

    Preparing a streak plate

    Preparing a spread plate

 

  Characteristic features of bacterial growth in broth and on agar

 

  Maintenance and storage of stock cultures

 

  Culturing anaerobic bacteria

 

Unit III — Visualizing bacteria

 

  Effective use and responsible care of the light microscope

 

  Measuring microscopic cells

 

  Preparing a hanging-drop slide

 

  Preparing a bacterial smear

 

  Preparing a simple stain

 

  Preparing a Gram stain

 

  Preparing an acid-fast stain

 

  Preparing a negative stain

 

  Preparing a capsule stain

 

  Preparing an endospore stain

 

Unit IV — Enzyme-based tests for the identification of bacteria

 

  Hydrolytic (digestive) enzymes

    Starch hydrolysis

    Casein hydrolysis

    Gelatin hydrolysis

    Lipid hydrolysis (tributyrin and spirit blue agar)

    DNA hydrolysis

  Utilization of carbohydrates

    Fermentation of carbohydrates (Durham tube)

    Methyl red test (mixed fermentation)

    Voges-Proskauer test  (butanediol fermentation)

    Citrate utilization

    Oxidation-fermentation (O-F) glucose test

 

  Degradat

Published by Pearson (October 24th 2006) - Copyright © 2007