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Anatomy & Physiology: Chemistry of Life and Biochemistry

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  • What is biochemistry?

    Biochemistry is the study of molecules that compose living organisms, including carbohydrates, fats, proteins, and nucleic acids.

  • What are electrolytes and their importance?

    Electrolytes are substances that dissociate in water to carry electrical current, essential for fluid balance, nerve impulses, muscle contraction, and heart health.

  • Define free radicals and antioxidants.


    Free radicals are unstable, reactive particles that can damage molecules; antioxidants neutralize free radicals to protect the body.

  • Why is water called the universal solvent?

    Water dissolves or suspends many chemicals in the body, making it essential for metabolism, lubrication, temperature regulation, and plasma composition.

  • What distinguishes solutions, colloids, and suspensions?

    Solutions have solute particles under 1 nm and are clear; colloids have particles 1–100 nm, scatter light, and are cloudy; suspensions have particles over 100 nm and separate on standing.

  • What is the pH scale and its physiological relevance?

    The pH scale measures acidity: 7 is neutral, below 7 acidic, above 7 basic. Blood pH is tightly regulated between 7.35-7.45 for normal function.

  • Define potential and kinetic energy in the body context.

    Potential energy is stored energy like ATP; kinetic energy is energy of motion used in muscle movement and blood flow, producing heat as a byproduct.

  • What are anabolism and catabolism?

    Anabolism builds molecules; catabolism breaks down molecules. Both are key metabolic processes.

  • What are monomers and polymers in biochemistry?

    Monomers are single subunits; polymers are chains of monomers, e.g., amino acids form proteins, nucleotides form nucleic acids.

  • List the four major organic macromolecules.

    Carbohydrates, lipids, proteins, and nucleic acids are the four major organic macromolecules essential for body structure and function.

  • What is the general formula and key feature of carbohydrates?

    Carbohydrates have a 2:1 hydrogen to oxygen ratio, are hydrophilic, and include sugars like glucose, the main energy source.

  • Name three important carbohydrate polysaccharides and their roles.

    Glycogen stores energy in animals; starch stores energy in plants; cellulose provides dietary fiber and plant structure.

  • What are lipids and their primary functions?

    Lipids are hydrophobic molecules used for long-term energy storage, insulation, and cell membrane structure.

  • Differentiate saturated and unsaturated fatty acids.

    Saturated fatty acids have single bonds and are solid at room temperature; unsaturated fatty acids have double bonds and are liquid oils.

  • What are triglycerides and their functions?

    Triglycerides consist of three fatty acids linked to glycerol, serving as energy storage, insulation, and shock absorption.

  • What roles do cholesterol and eicosanoids play?

    Cholesterol stabilizes cell membranes and is a hormone precursor; eicosanoids act as signaling molecules in inflammation and blood clotting.

  • Describe the general structure of an amino acid.

    An amino acid has a central carbon attached to an amino group, a carboxyl group, and a variable R group determining its properties.

  • What determines protein function and structure?

    Protein function depends on its unique three-dimensional conformation, which can be altered reversibly or irreversibly by denaturation.

  • List the four levels of protein structure.

    Primary (amino acid sequence), secondary (alpha helices and beta sheets), tertiary (3D folding), and quaternary (multiple polypeptide chains).

  • What is the role of enzymes in biochemical reactions?


    Enzymes are protein catalysts that lower activation energy, speeding up reactions without being consumed.

  • How do temperature and pH affect enzyme activity?

    Enzymes function best within narrow temperature and pH ranges; extremes can denature enzymes and reduce activity.

  • What is ATP and its significance?

    ATP is the energy currency of the cell, a nucleotide that stores and transfers energy for cellular work.

  • What are nucleotides and their components?

    Nucleotides consist of a nitrogenous base, a sugar, and a phosphate group; they are monomers of nucleic acids.

  • What is the difference between DNA and RNA?

    DNA is double-stranded and stores genetic information; RNA is single-stranded and helps translate DNA into proteins.

  • Name the four nitrogen bases in DNA and their pairing rules.

    Adenine pairs with Thymine; Cytosine pairs with Guanine in DNA's double helix structure.