Skip to main content
Back

Microscope and Microscopy Basics - Anatomy & Physiology

Control buttons has been changed to "navigation" mode.
1/18

Terms in this set (18)

  • What are the two main types of microscopes mentioned?
    Compound microscope and Dissecting (stereoscope) microscope.
  • How should you carry a microscope safely?
    Carry with two hands: one supporting the base and the other holding the arm.
  • What type of paper should be used to clean microscope lenses?
    Use lens paper to clean the lenses.
  • Which objective lens should you start with when viewing a specimen?
    Always begin with the lowest-power objective lens.
  • Which adjustment knob is used first to bring an object into focus?
    Use the coarse adjustment knob first, then the fine adjustment knob for sharp focus.
  • When is it appropriate to use only the coarse adjustment knob?
    Use only the coarse adjustment knob when using the scanning objective (4X).
  • Why should you keep both eyes open when using a microscope?
    To reduce eyestrain.
  • How does the field of view change with increasing magnification?
    The field of view gets smaller as magnification increases.
  • What is the formula to calculate the unknown diameter of the field of view?
    M1 × D1 = M2 × D2, where M is magnification and D is diameter of the field.
  • What is the diameter of the field of view at scanning power (4X)?
    4.7 mm or 4700 microns.
  • What is the diameter of the field of view at low power (10X)?
    1.8 mm or 1800 microns.
  • What is the diameter of the field of view at high power (43X)?
    0.5 mm or 500 microns.
  • Define Depth of Field (DOF) in microscopy.
    DOF is the depth of the area in acceptable focus or thickness of the plane clearly in focus.
  • How does Depth of Field change with magnification?
    Depth of Field decreases as magnification increases.
  • What is depth perception in microscopy?
    Seeing different planes of an object clearly by adjusting focus at different levels.
  • What is the purpose of using a coverslip in wet mount preparations?
    To protect the specimen and keep it flat for viewing.
  • What stain is used to view epithelial cheek cells under the microscope?
    Methylene blue stain.
  • What type of cells are shown in the cheek cavity surface view at 600× magnification?
    Epithelial cells of the cheek cavity.