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Microscope Basics and Techniques in Anatomy & Physiology

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Terms in this set (13)

  • Substage light

    What delivers a concentrated beam of light to the specimen?

  • What are the total magnifications for the 4x, 10x, 40x, and 100x objective lenses?
    4x lens = \(40\)×, 10x lens = \(100\)×, 40x lens = \(400\)×, 100x lens = \(1000\)× total magnification.
  • What is the proper procedure for carrying a microscope?
    Always use two hands, holding the microscope upright by the arm (frame) and supporting the base.
  • What should be used to clean microscope lenses?
    Use special grit-free lens paper to clean microscope lenses.
  • What is the purpose of stage clips on a microscope?
    Stage clips hold the specimen slides securely in place during observation.
  • Describe the focusing sequence from scanning to 400× magnification.
    Start with 4x objective using coarse and fine focus, adjust light and center specimen; 10x objective use coarse and fine focus; 40x objective use fine focus only and adjust light.
  • What are the steps to prepare a wet mount slide?
    Place a water droplet on a slide, add specimen into droplet with proper orientation, place cover slip at an angle, and add more water if needed.
  • Define parfocal in microscopy.
    Parfocal means once an object is in focus on one objective, switching objectives keeps the image nearly in focus.
  • What is resolution in microscopy?
    Resolution is the clarity or sharpness of an image.
  • What does contrast mean in microscopy?
    Contrast is the difference in brightness and color between the specimen and the background.
  • How does field of view change as magnification increases?
    As magnification increases, the field of view decreases.
  • If the microscope field diameter is 4.0 mm under scanning objective, what is it under low power?
    The field diameter under low power is approximately \(30\ mm\).
  • How can field diameter be used to estimate specimen size?
    If a specimen is about half the field diameter, its size can be estimated as half the field diameter length.