Which type of reaction is involved in peptide bond formation, and what does it require?

How do dehydration synthesis and hydrolysis differ in terms of energy changes and their effects on peptide bonds?

Evaluate the factors that contribute to the stability of peptide bonds in proteins.

If a protein's primary structure is altered, what potential effects might this have on the protein's stability?

How might a change in the primary structure affect a protein's enzymatic activity?

Which of the following best describes the primary structure of a protein?

What is the difference between changing amino acid composition and sequence?

If the amino acids in a protein are rearranged without changing the composition, what is the likely effect?

Which amino acid substitution is most likely to maintain protein function?

Which of the following statements correctly describes the ionizable groups in a peptide chain?

What is the final step in drawing a peptide structure?

Why is it crucial to correctly identify the NCC sequence in peptide drawing?

Given a peptide with the following ionizable groups: N-terminus (pKa 8.0), C-terminus (pKa 3.1), and a glutamic acid side chain (pKa 4.1), what is the net charge at pH 7.4?

What happens to the charge of an ionizable group when the pH is higher than its pKa?