Biochemistry
What does Vmax represent in enzyme kinetics?
Given the Michaelis-Menten equation V0 = (Vmax[S])/(Km + [S]), how would you rearrange it to solve for Vmax?
Using the Lineweaver-Burk equation 1/V0 = (Km/Vmax)(1/[S]) + 1/Vmax, how can you solve for Vmax?
Under saturating substrate conditions, how does the initial reaction velocity (V0) relate to Vmax?
What is the rate law for the product formation step in enzyme-catalyzed reactions?
If V0 = 10 μM/s, Km = 5 μM, and [S] = 20 μM, what is Vmax using the Michaelis-Menten equation?
In a reaction where V0 is measured at saturating substrate concentrations, what substitution can be made in the rate law?
