What does the acronym ELISA stand for?

Which of the following diseases can be diagnosed using ELISA?

Why is the enzyme-linked secondary antibody crucial in the ELISA process?

Why is sandwich ELISA considered more sensitive and specific than indirect ELISA?

What is a major challenge in selecting antibodies for a sandwich ELISA?

Why is blocking unoccupied sites with non-specific proteins crucial in ELISA?

How does spectrophotometry contribute to the quantification of antigens in ELISA?