How does increasing temperature affect enzyme-catalyzed reaction rates, and what is a potential risk of high temperatures?

A cell is attempting to increase the rate of a reaction by increasing substrate concentration. What potential issue might arise from this approach?

What does the total enzyme concentration (ET) represent in enzyme kinetics?

Which constant in enzyme kinetics is used to describe the turnover number of an enzyme?

In a laboratory experiment, what is a potential consequence of continuously increasing substrate concentration?

What does the variable 'ES' represent in enzyme kinetics?

In an enzyme-catalyzed reaction, what does vmax represent?