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Why is FDNB sometimes referred to as Sanger's reagent?
A protein with three subunits is treated with FDNB. How many N-terminal residues will be labeled?
How can the analysis of DNP derivatives using HPLC help determine the number of subunits in a protein?
Which of the following reagents is used similarly to FDNB for labeling N-terminal amino acids?
In an HPLC chromatogram, how can you distinguish between peaks corresponding to DNP derivatives and free amino acids?
A protein treated with FDNB shows two DNP peaks in HPLC analysis. What does this indicate about the protein?
If a protein with four subunits is treated with FDNB, how many DNP derivatives should be expected after hydrolysis?