Biochemistry
Which type of ion-exchange chromatography is used to purify negatively charged proteins?
In cation exchange chromatography, what type of charge does the stationary phase carry, and why is it important?
How does cation exchange chromatography utilize the stationary resin to separate proteins based on charge?
Why is salt added to a cation exchange chromatography column to elute positively charged proteins?
How does the magnitude of a protein's charge affect its movement through a cation exchange chromatography column?
Why is achieving good separation in cation exchange chromatography crucial for purifying target proteins?
Given proteins with net charges of -3, +1, and 0, which will elute first from a cation exchange chromatography column?
