Biochemistry
Why do more acidic amino acids have more acidic isoelectric points?
Calculate the isoelectric point of glycine given pKa1 = 2.34 and pKa2 = 9.60.
What role does pH play in adjusting molecular charge?
How does adjusting the pH to the isoelectric point aid in protein separation during electrophoresis?
What is the effect of reaching the isoelectric point on a molecule's migration in an electric field?
Calculate the isoelectric point of threonine given pKa1 = 2.09 and pKa2 = 9.10.
What makes the isoelectric point a useful property for protein isolation?