Biochemistry
What is the isoelectric point of a peptide?
Why is it important to use pKa values specific to amino acid residues when calculating the isoelectric point of a peptide?
A peptide has pKa values of 3.0, 6.0, and 9.0. What is the net charge at pH 7.0?
Calculate the isoelectric point for a peptide with pKa values of 3.1, 5.5, and 8.2, where the neutral net charge occurs between 5.5 and 8.2.
Given a peptide sequence of K-R-E and pKa values of 2.2, 9.0, 10.5, and 12.5, estimate the isoelectric point.
A peptide has pKa values of 2.0, 4.5, and 9.0. At pH 5.0, the net charge is -1. What does this indicate about the predominant form of the peptide?
Which two pKa values would you average to find the isoelectric point if the neutral net charge occurs between pKa values of 3.0 and 5.0?
