Why is the initial velocity assumption important in enzyme kinetics?

Why is the reverse reaction considered negligible during the initial velocity phase?

Given an enzyme-catalyzed reaction with a high substrate concentration, which assumption allows you to use the Michaelis-Menten equation?

How does the concept of the enzyme-substrate complex in the Michaelis-Menten model differ from earlier theories?

Evaluate the validity of using the free substrate concentration to represent the total substrate concentration in the Michaelis-Menten equation.

In a reaction where the reverse reaction is negligible, what can be inferred about the product concentration?

Evaluate the effectiveness of using the Michaelis-Menten equation in a reaction with low substrate concentration.