Biochemistry
Which of the following best describes the process of preparing large proteins for Edmond degradation sequencing?
Why is it necessary to use multiple cleavage techniques to accurately determine the sequence of a large protein?
Given two sets of peptide fragments from different cleavage techniques, how would you determine the original protein sequence?
What is the first step in preparing a large protein for sequencing using Edmond degradation?
A protein is cleaved with cyanogen bromide, resulting in three fragments. Which of the following statements is true?
What is the benefit of using both cyanogen bromide and chymotrypsin in protein sequencing?
Which method is used to align overlapping fragments and confirm the sequence of the original protein?