Biochemistry
How does beta mercaptoethanol affect protein structure during SDS-PAGE?
A protein sample shows three bands on an SDS-PAGE gel. Which conclusion can be drawn about the protein's subunits?
What would be the expected outcome on an SDS-PAGE gel if a protein with disulfide-linked subunits is treated with beta mercaptoethanol?
How do covalent and non-covalent interactions affect protein migration in SDS-PAGE?
Which factor is NOT a determinant of protein migration in native PAGE?
How can the use of SDS-PAGE with beta mercaptoethanol help determine the quaternary structure of a protein?
A protein sample shows bands at 150 kDa, 75 kDa, and 50 kDa on an SDS-PAGE gel. What can be inferred about the protein?
