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Anatomy & Physiology: Cell Biology, Tissues, and Molecular Processes

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  • DNA

    DNA is the molecule that stores genetic information in cells. It consists of nucleotide sequences forming double-stranded helices and serves as the template for transcription.

  • mRNA

    mRNA (messenger RNA) is a single-stranded RNA that carries the genetic code transcribed from DNA to ribosomes for protein synthesis.

  • tRNA

    tRNA (transfer RNA) brings specific amino acids to the ribosome during translation by matching its anticodon to mRNA codons.

  • DNA triplets, mRNA codons, and tRNA anticodons

    DNA triplets are three-nucleotide sequences in DNA; mRNA codons are complementary three-nucleotide sequences transcribed from DNA; tRNA anticodons are complementary to mRNA codons and ensure correct amino acid placement during translation.

  • Helicase

    Helicase unwinds the DNA double helix during transcription, separating the strands to allow RNA polymerase access.

  • RNA polymerase

    RNA polymerase synthesizes mRNA by reading the DNA template strand during transcription.

  • Promotor

    The promotor is a DNA sequence where RNA polymerase binds to initiate transcription.

  • Start codon

    The start codon (usually AUG) signals the beginning of translation and codes for methionine.

  • Stop codon

    Stop codons (UAA, UAG, UGA) signal the end of translation, terminating polypeptide synthesis.

  • Spliceosomes

    Spliceosomes are complexes that remove introns from pre-mRNA, joining exons to form mature mRNA.

  • Introns and Exons

    Introns are non-coding sequences removed from pre-mRNA; exons are coding sequences joined to form mature mRNA.

  • Ribosome A, P, and E sites

    The A site binds incoming tRNA; the P site holds the tRNA with the growing polypeptide; the E site is where tRNA exits after delivering its amino acid.

  • Golgi apparatus role in translation

    The Golgi apparatus modifies, folds, and packages proteins made by ribosomes for export or use within the cell.

  • Nucleotide and nitrogenous base

    A nucleotide is the building block of DNA/RNA, consisting of a sugar, phosphate, and nitrogenous base; the nitrogenous base encodes genetic information.

  • Structural differences between DNA and mRNA

    DNA is double-stranded with deoxyribose sugar; mRNA is single-stranded with ribose sugar and uracil instead of thymine.

  • Functional uses of DNA vs RNA types

    DNA stores genetic info; mRNA carries code to ribosomes; rRNA forms ribosome structure; tRNA delivers amino acids during translation.

  • Relationship between DNA triplet, mRNA codon, and tRNA anticodon

    DNA triplets are transcribed into complementary mRNA codons, which are matched by tRNA anticodons during translation to add correct amino acids.

  • Dehydration synthesis in polypeptide formation

    Amino acids are joined by dehydration synthesis, forming peptide bonds and releasing water to build polypeptide chains.

  • Example: DNA triplets AAT—CCA—GGT to mRNA codons and tRNA anticodons

    DNA triplets AAT—CCA—GGT transcribe to mRNA codons UUA—GGU—CCA and pair with tRNA anticodons AAU—CCA—GGU.

  • Amino acid and protein

    Amino acids are building blocks of proteins, which are chains of amino acids folded into functional molecules.

  • Gene and genome

    A gene is a DNA segment coding for a protein; the genome is the complete set of genes in an organism.

  • Cytoplasm vs cytosol

    Cytoplasm includes cytosol plus organelles; cytosol is the gel-like fluid inside cells where organelles are suspended.

  • Simple squamous epithelium

    Flat cells with flattened nuclei; found in lung alveoli and capillaries for diffusion of gases and nutrients.

  • Simple cuboidal epithelium

    Cube-shaped cells with round nuclei; found in glands and kidney tubules for secretion and absorption.

  • Simple columnar epithelium

    Tall, column-shaped cells with oval nuclei; found in intestines for nutrient absorption and mucus secretion.

  • Transitional epithelium

    Layered cells that stretch from many layers to one; found in urinary bladder to accommodate volume changes.

  • Skeletal muscle tissue

    Long, multinucleate, striated cells under voluntary control; attached to bones for movement.

  • Cardiac muscle tissue

    Striated, branched cells with intercalated disks; involuntary control for heart contraction.

  • Smooth muscle tissue

    Spindle-shaped, non-striated cells with gap junctions; involuntary control in hollow organs for movement of contents.