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Microbiology Fundamentals 1-4

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  • Phagocytosis

    Phagocytosis is the ingestion of bacteria or other material by phagocytes and amoeboid protozoans.

  • Pinocytosis

    Pinocytosis is the ingestion of liquid into a cell by the budding of small vesicles from the cell membrane.

  • Exocytosis

    Exocytosis is the process where cell vacuole contents are released outside the cell by fusion of the vacuole membrane with the cell membrane.

  • Lysosomes

    Lysosomes are organelles containing digestive enzymes that digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria.

  • Centrosome (Centrioles)

    The centrosome organizes microtubules, provides cell structure, and pulls chromatids apart during cell division.

  • Chloroplasts

    Chloroplasts convert sunlight into sugars usable by cells through photosynthesis.

  • Nucleus

    The nucleus controls and regulates cell activities like growth and metabolism and contains hereditary genetic information.

  • Endoplasmic Reticulum

    The endoplasmic reticulum produces and processes proteins and lipids; it is a series of sacs and tubular folds outside the nucleus.

  • Ribosomes (70S vs 80S)

    Ribosomes synthesize proteins; 70S ribosomes are found in prokaryotes, 80S in eukaryotes.

  • Golgi Complex

    The Golgi complex processes and packages macromolecules like proteins and lipids synthesized by the cell.

  • Mitochondria

    Mitochondria perform cellular respiration, breaking down nutrients to produce energy.

  • Differences between Eukaryotic and Prokaryotic Cells

    Eukaryotic cells have membrane-bound organelles including a nucleus; prokaryotic cells lack a nucleus and membrane-bound organelles.

  • Endospore Formation

    Endospore formation occurs under nutrient scarcity in gram-positive bacteria, producing dormant spores that can survive long periods.

  • Cytoplasm

    Cytoplasm is a thick solution filling the cell, supporting and suspending organelles and molecules.

  • Plasma Membrane Structure

    The plasma membrane is a phospholipid bilayer with embedded proteins, selectively permeable to regulate substance movement in and out of cells.

  • Osmosis

    Osmosis is the diffusion of water across a selectively permeable membrane from low to high solute concentration.

  • Facilitated Diffusion

    Facilitated diffusion is the movement of particles down their concentration gradient with the help of carrier molecules, without energy use.

  • Active Transport

    Active transport moves substances from low to high concentration across membranes, requiring energy.

  • Gram-Positive vs Gram-Negative Cell Walls

    Gram-positive bacteria have thick peptidoglycan layers and teichoic acids; Gram-negative bacteria have thin peptidoglycan, an outer membrane with lipopolysaccharides (LPS), and a periplasmic space.

  • Gram Stain Steps

    1. Apply crystal violet (primary stain). 2. Add Gram's iodine (mordant). 3. Decolorize with ethanol/acetone. 4. Counterstain with safranin.

  • Capsule (Glycocalyx)

    The capsule is a carbohydrate-rich coating outside many cells, protecting and helping bacteria attach to surfaces.

  • Flagella

    Flagella are whip-like structures that enable cell movement.

  • Pili

    Pili are thin, rigid protein fibers that help bacteria attach to surfaces or other cells.

  • Axial Filaments

    Axial filaments are rotating endo-flagella that allow spirochetes to move in a corkscrew motion.

  • Bacterial Shapes: Coccus, Bacillus, Spiral

    Coccus is spherical, Bacillus is rod-shaped, and spiral includes vibrio (comma-shaped), spirillum, and spirochete forms.

  • Binary Fission

    Binary fission is asexual reproduction where a cell duplicates its DNA and divides into two identical cells.

  • Three Domains of Life

    Bacteria and Archaea are prokaryotic without nuclei; Eukarya have nuclei and membrane-bound organelles.

  • Five Kingdoms

    1. Monera (prokaryotes), 2. Protista (single/multicellular eukaryotes), 3. Fungi (non-photosynthetic decomposers), 4. Animalia (multicellular heterotrophs), 5. Plantae (mostly autotrophic eukaryotes).

  • Germ Theory

    Germ theory states that microorganisms cause disease, disproving spontaneous generation; developed by Louis Pasteur.