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General Biology: Metabolism, Cellular Respiration, and Photosynthesis

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  • What is metabolism?

    Metabolism is the sum of all chemical reactions that occur within a cell or organism to maintain life, including catabolic and anabolic pathways.

  • Define catabolic and anabolic pathways.

    Catabolic pathways break down molecules to release energy, while anabolic pathways build complex molecules using energy.

  • What are the main forms of energy relevant to biology?

    Kinetic energy is energy of motion, thermal energy is heat, potential energy is stored energy, and chemical energy is energy stored in chemical bonds.

  • What is free energy and its significance?

    Free energy (G) is the energy available to do work in a system; changes in free energy determine if a reaction is spontaneous.

  • State the first and second laws of thermodynamics.

    The first law states energy cannot be created or destroyed. The second law states entropy (disorder) of the universe increases over time.

  • What is entropy?

    Entropy is a measure of disorder or randomness in a system, which tends to increase in spontaneous processes.

  • Difference between exergonic and endergonic reactions.

    Exergonic reactions release energy and are spontaneous; endergonic reactions require energy input and are non-spontaneous.

  • What is ATP and its role in energy coupling?

    ATP (adenosine triphosphate) stores and transfers energy; it couples exergonic and endergonic reactions by transferring phosphate groups.

  • What is a phosphorylated intermediate?

    A phosphorylated intermediate is a molecule with a phosphate group covalently bonded, making it more reactive in metabolic reactions.

  • How do enzymes lower activation energy?

    Enzymes lower the activation energy by stabilizing the transition state and providing an active site for substrates to bind.

  • What is feedback inhibition?

    Feedback inhibition is when the end product of a metabolic pathway inhibits an enzyme early in the pathway to regulate its own production.

  • What is the overall equation for cellular respiration?

    Cellular respiration: \(\mathrm{C_6H_{12}O_6 + 6O_2 \rightarrow 6CO_2 + 6H_2O + energy}\)

  • Define oxidation and reduction in redox reactions.

    Oxidation is loss of electrons; reduction is gain of electrons in redox reactions.

  • What occurs during glycolysis and its ATP yield?

    Glycolysis breaks glucose into two pyruvate molecules, producing a net gain of 2 ATP and 2 NADH molecules.

  • What is Acetyl CoA and its role?

    Acetyl CoA is formed from pyruvate oxidation and enters the citric acid cycle to be further oxidized for energy extraction.

  • What is the citric acid cycle's starting molecule?

    The cycle starts with Acetyl CoA combining with oxaloacetate to form citrate.

  • Where does oxidative phosphorylation occur and what is its purpose?

    It occurs in the inner mitochondrial membrane and produces ATP using the electron transport chain and chemiosmosis.

  • What is the role of oxygen in the electron transport chain?

    Oxygen acts as the final electron acceptor, combining with electrons and protons to form water.

  • Difference between NAD+ and NADH states.

    NAD+ is the oxidized form; NADH is the reduced form carrying electrons after glycolysis and the citric acid cycle.

  • What happens during fermentation and its ATP yield?

    Fermentation regenerates NAD+ from NADH allowing glycolysis to continue, producing 2 ATP per glucose without oxygen.

  • What occurs during the light reactions of photosynthesis?

    Light reactions convert light energy into chemical energy (ATP and NADPH) and release oxygen from water.

  • What is the Calvin cycle and why is it called a dark reaction?

    The Calvin cycle fixes carbon dioxide into sugars using ATP and NADPH; it is called a dark reaction because it does not require light directly.

  • What are photosystems and their role?

    Photosystems are protein-pigment complexes that capture light energy to drive electron transport in photosynthesis.

  • What is a photon?

    A photon is a particle of light that carries energy used in photosynthesis.