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Starch Acid Hydrolysis Experiment - General Biology

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  • What carbohydrate is present at the beginning of the starch acid hydrolysis experiment?

    Starch is the carbohydrate present at the beginning (unhydrolyzed).

  • What carbohydrate is present at the end of the starch acid hydrolysis experiment?

    Glucose (or reducing sugar/maltose) is present at the end (hydrolyzed).

  • What causes starch to break down during acid hydrolysis?

    Acid and heat break down starch polysaccharide chains step-by-step into smaller sugar units (monosaccharides/reducing sugars).

  • Which reagent tests positive at the beginning of the starch acid hydrolysis experiment?

    IKI (Iodine) test detects starch and tests positive at the beginning.

  • Which reagent tests negative at the beginning of the starch acid hydrolysis experiment?

    Benedict’s test tests negative at the beginning because no reducing sugar is present yet.

  • Which reagent tests positive at the end of the starch acid hydrolysis experiment?

    Benedict’s test detects newly formed reducing sugars and tests positive at the end.

  • Which reagent tests negative at the end of the starch acid hydrolysis experiment?

    IKI test tests negative at the end because starch is fully hydrolyzed and used up.

  • What type of sugars are produced from starch hydrolysis?

    Monosaccharides or reducing sugars such as glucose and maltose are produced.

  • What is the role of heat in starch acid hydrolysis?

    Heat accelerates the breakdown of starch polysaccharides into smaller sugar units.

  • Why does Benedict’s test become positive after starch hydrolysis?

    Because reducing sugars like glucose are formed, which react with Benedict’s reagent.