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Chemical Principles in Microbiology

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  • What is an atom?

    An atom is the smallest unit of matter that cannot be subdivided into smaller substances.

  • What particles compose an atom?

    Atoms are composed of electrons (negative), protons (positive), and neutrons (neutral). Protons and neutrons form the nucleus, electrons orbit around it.

  • What defines a chemical element?

    Atoms with the same number of protons are classified as the same chemical element.

  • What is the atomic number?

    The atomic number is the number of protons in the nucleus of an atom.

  • How are electrons arranged in an atom?

    Electrons are arranged in electron shells corresponding to different energy levels; the innermost shell has the lowest energy.

  • What is valence in atoms?

    Valence is the number of missing or extra electrons in the outermost electron shell of an atom.

  • How do atoms form molecules?

    Atoms form molecules by filling their outermost shells through chemical bonds formed by valence electrons.

  • What are the main types of chemical bonds?

    Ionic bonds form by electron transfer; covalent bonds form by sharing electrons; hydrogen bonds are weak attractions involving hydrogen and electronegative atoms.

  • What is an ionic bond?

    An ionic bond is an attraction between oppositely charged ions formed when atoms gain or lose electrons.

  • What is a covalent bond?

    A covalent bond forms when two atoms share one or more pairs of electrons; it is stronger and more common in organisms than ionic bonds.

  • What is a hydrogen bond?

    A hydrogen bond is a weak interaction where a hydrogen atom covalently bonded to an electronegative atom is attracted to another electronegative atom.

  • What are synthesis reactions?

    Synthesis reactions combine atoms or molecules to form larger molecules; they are anabolic and include dehydration synthesis.

  • What are decomposition reactions?

    Decomposition reactions break down molecules into smaller parts; they are catabolic and include hydrolysis.

  • What are exchange reactions?

    Exchange reactions involve both synthesis and decomposition, where bonds are both made and broken.

  • What are redox reactions?

    Redox reactions involve the transfer of electrons; oxidation is loss of electrons, reduction is gain of electrons.

  • What distinguishes inorganic from organic compounds?

    Inorganic compounds usually lack carbon and are simple; organic compounds contain carbon and are structurally complex.

  • What is the carbon skeleton in organic molecules?

    The carbon skeleton is the chain of carbon atoms in an organic molecule, which can form straight, branched, or ring structures.

  • What are functional groups in organic chemistry?

    Functional groups are specific groups of atoms attached to carbon skeletons that determine chemical properties of organic compounds.

  • What are macromolecules?

    Macromolecules are large molecules formed by covalent bonding of many monomers; examples include carbohydrates, lipids, proteins, and nucleic acids.

  • What is dehydration synthesis?

    Dehydration synthesis joins two monomers by removing a water molecule, forming a covalent bond.

  • What are carbohydrates?

    Carbohydrates serve as cell structures and energy sources; they include sugars and starches composed of C, H, and O.

  • What are monosaccharides?

    Monosaccharides are simple sugars with 3-7 carbon atoms, e.g., glucose and deoxyribose.

  • What are disaccharides?

    Disaccharides form when two monosaccharides join by dehydration synthesis and can be broken down by hydrolysis.

  • What are lipids?

    Lipids are nonpolar molecules mainly composed of C, H, and O; they include fats (triglycerides) and complex lipids like phospholipids.

  • What is the difference between saturated and unsaturated fats?

    Saturated fats have no double bonds in fatty acids; unsaturated fats have one or more double bonds.

  • What are phospholipids?

    Phospholipids are complex lipids with a glycerol, two fatty acids, and a phosphate group; they have polar heads and nonpolar tails important for cell membranes.

  • What are proteins?

    Proteins are made of amino acids and are essential for cell structure, enzymes, transport, movement, and toxins.

  • What are amino acids?

    Amino acids are protein subunits containing an alpha-carbon with attached amino, carboxyl, and side groups.

  • What are the levels of protein structure?

    Protein structure levels include primary (amino acid sequence), secondary (helix and sheets), tertiary (3D folding), and quaternary (multiple chains).

  • What is denaturation of proteins?

    Denaturation is the loss of protein shape and function due to hostile environments like temperature or pH changes.

  • What are nucleic acids?

    Nucleic acids consist of nucleotides made of a pentose sugar, phosphate group, and nitrogenous base; DNA and RNA are examples.

  • What is ATP?

    Adenosine triphosphate (ATP) stores chemical energy and releases it by hydrolysis of phosphate groups for cellular use.