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Introduction to Microbiology

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  • What is microbiology?

    Microbiology is the study of microbes, including organisms and infectious agents too small to be seen with the naked eye.

  • Define microorganism and microbe.

    Microorganism: living organism too small to see with the naked eye.
    Microbe: includes microorganisms and non-living infectious agents too small to see.

  • Who first visualized microorganisms and what did he call them?

    Antonie van Leeuwenhoek first observed microorganisms in 1674 and called them animalicules (little animals).

  • What is taxonomy?

    Taxonomy is the branch of science that classifies, identifies, and names organisms using hierarchical categories.

  • Name the three domains of life.

    The three domains are Bacteria, Archaea, and Eukarya.

  • What distinguishes prokaryotes from eukaryotes?

    Prokaryotes are unicellular organisms without a nucleus; eukaryotes have cells with a nucleus and can be unicellular or multicellular.

  • Which kingdoms belong to the domain Eukarya?

    Kingdoms in Eukarya include Animalia, Plantae, Fungi, and Protista.

  • How are organisms categorized based on energy acquisition?

    Organisms are categorized as autotrophs (make their own food), heterotrophs (consume others), or decomposers (consume dead matter).

  • Describe the binomial naming system.

    Developed by Carl Linnaeus, it uses two Latin parts: Genus (capitalized) and species (lowercase), both italicized or underlined.

  • What are strains in microbiology?

    Strains are genetic variants within a species, showing minor differences.

  • What are bacteria?

    Bacteria are unicellular, prokaryotic organisms with cell walls made of peptidoglycan, reproducing by binary fission.

  • What are archaea and how do they differ from bacteria?

    Archaea are prokaryotes with unique rRNA sequences and cell walls lacking peptidoglycan; many are extremophiles.

  • What defines eukaryotes?

    Eukaryotes have membrane-bound nuclei and include fungi, algae, protozoa, and helminths.

  • What are fungi?

    Fungi are eukaryotes ranging from unicellular yeasts to multicellular molds; they have chitin cell walls and do not perform photosynthesis.

  • What are algae?

    Algae are photosynthetic eukaryotes, unicellular or multicellular, with cellulose cell walls, found in aquatic or moist habitats.

  • What are protozoa?

    Protozoa are unicellular eukaryotes without cell walls, often motile and ingest organic material for food.

  • What are helminths?

    Helminths are parasitic worms; not microscopic but their eggs and larvae are, so they are studied in microbiology.

  • What are acellular infectious agents?

    Acellular infectious agents like viruses, viroids, and prions lack cellular structure and cannot reproduce independently.

  • What are viruses?

    Viruses are obligate intracellular parasites made of DNA or RNA inside a protein coat; they infect all life domains.

  • What are viroids?

    Viroids are small, circular, single-stranded RNA molecules that infect plants and cause disease.

  • What are prions?

    Prions are misfolded proteins that cause normal proteins to misfold, leading to neurodegenerative diseases.

  • Why are microorganisms important commercially?

    Microorganisms are used to produce food (bread, beer, yogurt), antibiotics, biofuels, insecticides, and plastics.

  • What environmental roles do microorganisms play?

    They fix nitrogen, degrade cellulose, and perform bioremediation to clean pollutants.

  • Why are microorganisms useful as research tools?

    They share fundamental metabolic and genetic features with complex organisms and grow quickly and inexpensively.

  • What is the human microbiota?

    The normal microbiota are trillions of microbes living on and in humans, aiding health by competing with pathogens.

  • What is the scientific method?

    A procedure to ask questions, test ideas, and build scientific knowledge through observation, hypothesis, experimentation, and analysis.

  • Define hypothesis, prediction, and theory.

    Hypothesis: testable explanation.
    Prediction: expected outcome.
    Theory: well-supported, testable explanation.

  • What are independent and dependent variables?

    Independent variable: factor changed by the experimenter.
    Dependent variable: factor measured or observed.

  • What are false positives and false negatives?

    False positive: incorrect indication of a positive result.
    False negative: incorrect indication of a negative result.

  • What are positive and negative controls?

    Positive control: group expected to show a positive result.
    Negative control: group expected to show no effect.