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Microbiology: Bacterial Growth and Physiology
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Why does bacterial growth decrease above the maximum growth temperature?
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Why does bacterial growth decrease above the maximum growth temperature?
Enzymes and proteins become
denatured
, impairing cellular functions.
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Why does bacterial growth decrease above the maximum growth temperature?
Enzymes and proteins become
denatured
, impairing cellular functions.
Which bacterial growth phase shows decreasing viable cell numbers due to nutrient exhaustion and waste accumulation?
The
death phase
.
Do all microorganisms in biofilms belong to the same species?
No, biofilms often contain
multiple species
and organisms from different kingdoms.
What environment favors growth of a bacterium that grows best at pH 9.5?
An
alkaline lake
.
What is the effect of a drug that prevents FtsZ ring formation in bacteria?
Cytokinesis cannot occur
, preventing cell division.
A microorganism requiring at least 9% NaCl for growth is classified as a:
Extreme halophile
.
Which bacterial growth phase is metabolically active but shows no increase in cell number?
The
stationary phase
.
What component primarily holds a biofilm together?
The
EPS (extracellular polymeric substance) matrix
.
How can growth of a foodborne pathogen be reduced without antimicrobials?
By
reducing water availability and lowering temperature
.
Why are jams and jellies resistant to microbial spoilage?
High sugar content
reduces water availability
.
What does continued bacterial growth despite oxygen depletion indicate?
The organism may be capable of
anaerobic metabolism
.
Which bacterial growth phase occurs when a culture reaches carrying capacity?
The
stationary phase
.
Why is infection by a hyperthermophile unlikely in the human body?
Its enzymes are optimized for
much higher temperatures
than human body temperature.
How is a bacterium growing best at pH 2.5 classified?
As an
acidophile
.
During which bacterial growth phase do patients often first develop symptoms?
During the
log (exponential) phase
.
A bacterium growing only at the top of a thioglycolate tube is classified as:
An
obligate aerobe
.
Which group likely causes growth of pathogens in refrigerated foods at 4°C?
Psychrotrophs
.
A bacterium growing evenly throughout a thioglycolate tube without using oxygen is:
An
aerotolerant anaerobe
.
What characteristic contributes most to biofilm resistance?
The
protective EPS matrix
.
What process occurs when a bacterium placed in concentrated brine immediately loses water?
Plasmolysis
.
Why are obligate anaerobes often harmed by oxygen?
They lack enzymes that
detoxify reactive oxygen species
.
A microorganism growing optimally at 4°C and not above 20°C is a:
Psychrophile
.
What explains recurrent catheter-associated infections?
Development of
biofilms on the catheter surface
.
A bacterium growing at 4°C, 25°C, and 37°C is likely a:
Psychrotroph
, capable of growth during refrigeration.
Successful antibiotic treatment should push bacteria into which phase?
The
death phase
.
A chronic infection with persistent low-level symptoms resembles which bacterial phase?
The
stationary phase
.
Which process is affected if a mutant bacterium lacks functional Par proteins?
Chromosome segregation
.
What effect does a drug interfering with quorum sensing have?
It causes
reduced community coordination and virulence
.
If a bacterial species doubles every 30 minutes, how many cells after 2 hours starting from 1,000?
16,000 cells.
Most effective strategy to prevent biofilm formation on medical implants?
Prevent initial bacterial attachment
.
A pathogen synthesizing enzymes but not increasing in number is in which phase?
The
lag phase
.
During which phase are secondary metabolites like antibiotics commonly produced?
The
stationary phase
.
Why can strict anaerobes survive within dental plaque?
Surface organisms consume oxygen, creating
anaerobic niches
.
Why do generation times vary between bacterial species?
Generation time varies among species and environmental conditions.
A bacterial community attached to a surface with EPS and quorum sensing is a:
Biofilm
.
An organism growing with oxygen but switching to anaerobic respiration is a:
Facultative anaerobe
.
Why are most human pathogens neutrophiles?
Because human body fluids are near
neutral pH
.
How does Helicobacter pylori survive stomach acidity?
It produces
urease
that raises local pH.
A drug inhibiting Fts proteins affects:
Cell division
.
During which phase are bacteria most susceptible to antibiotics?
During the
log (exponential) phase
.