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Microbiology Chapters 1-5 Exam Review

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  • Denaturation

    Loss of a protein's normal three-dimensional shape, often caused by high temperature or pH changes.

  • Proton

    Subatomic particle that determines an element's identity by its positive charge and atomic number.

  • Polarity

    Unequal distribution of electrical charge in a molecule, making molecules like water effective solvents.

  • Valence electron

    Electron in the outermost shell that influences chemical reactivity and bonding.

  • Isotope

    Atom of the same element with a different number of neutrons, affecting atomic mass but not chemical properties.

  • Covalent bond

    Bond formed when atoms share electrons to achieve stability.

  • Activation energy

    Minimum energy needed to begin a chemical reaction, often lowered by enzymes.

  • Hydrogen bond

    Weak attraction between molecules that helps produce many of water's unique properties.

  • Total magnification

    Product of ocular-lens power and objective-lens power, e.g., 10x ocular × 40x objective = 400x.

  • Resolution

    Ability to distinguish two nearby points as separate under a microscope.

  • Immersion oil

    Material used with the 100x objective to reduce refraction and increase resolution.

  • Brownian motion

    Random jittering caused by collisions with surrounding molecules, not true motility.

  • Aseptic technique

    Practice used to prevent contamination of cultures, workers, and the environment.

  • Gram stain decolorizer

    Reagent that removes dye from gram-negative cells, typically alcohol or acetone.

  • Basic dye

    Positively charged stain that colors bacterial cells by binding to negatively charged cell components.

  • Negative stain

    Dye repelled by bacterial cells that stains the background, preserving cell size and shape.

  • Coccus

    Spherical bacterial cell shape.

  • Bacillus

    Rod-shaped bacterial cell.

  • Streptococci

    Spherical bacterial cells arranged in chains.

  • Staphylococci

    Spherical bacterial cells arranged in irregular clusters.

  • Peptidoglycan

    Cell-wall polymer made from NAG and NAM sugars, providing structural support.

  • Gram-negative cell wall

    Thin peptidoglycan layer with an additional outer membrane containing lipopolysaccharide (LPS).

  • Teichoic acid

    Polymer found in gram-positive cell walls that provides rigidity and antigenic properties.

  • Mycolic acid

    Waxy cell-wall component responsible for acid-fastness in bacteria like Mycobacterium.

  • Aerobic respiration

    Pathway using oxygen as the final electron acceptor to produce ATP efficiently.

  • Glycolysis

    First pathway of glucose breakdown with net yield of 2 ATP and 2 NADH molecules.

  • Electron transport chain (ETC)

    Membrane series that passes electrons and pumps protons to create a proton gradient.

  • Chemiosmosis

    Proton movement down a gradient through a membrane protein to drive ATP synthesis.

  • ATP synthase

    Enzyme driven by proton flow to phosphorylate ADP into ATP.

  • Fermentation

    Pathway that regenerates NAD+ without using Krebs cycle or ETC, producing less ATP.