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Microbiology: Chemical Principles and Biological Molecules

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  • What is an atom?

    An atom is the smallest unit of a chemical element that retains the element's properties.

  • What particles make up an atom?

    Atoms contain protons (positive), neutrons (no charge), and electrons (negative).

  • What is the atomic number?

    The atomic number is the number of protons in an atom's nucleus and determines the element's identity.

  • What is atomic mass?

    Atomic mass is approximately the total number of protons and neutrons in an atom.

  • Define isotopes.

    Isotopes are atoms of the same element with the same number of protons but different numbers of neutrons and atomic masses.

  • What are valence electrons?

    Valence electrons are electrons in the outermost shell that determine chemical properties and reactivity.

  • Difference between molecule and compound?

    A molecule is two or more atoms joined chemically; a compound contains at least two different elements.

  • What is a chemical bond?

    A chemical bond is an attractive force holding atoms together to achieve stable electron configurations.

  • Describe ionic bonds.

    Ionic bonds form by electron transfer between atoms, creating oppositely charged ions that attract.

  • Describe covalent bonds.

    Covalent bonds form when atoms share pairs of electrons; they are stronger than ionic bonds.

  • What are hydrogen bonds?

    Hydrogen bonds are weak attractions involving a hydrogen covalently bonded to O or N, attracted to another O or N.

  • What is molecular mass?

    Molecular mass is the sum of the atomic masses of all atoms in a molecule.

  • Define endergonic and exergonic reactions.

    Endergonic reactions require energy input; exergonic reactions release energy.

  • What are synthesis, decomposition, and exchange reactions?

    Synthesis builds molecules; decomposition breaks molecules down; exchange swaps parts between molecules.

  • Why is water important in cells?

    Water is abundant, polar, an excellent solvent, participates in hydrolysis, and buffers temperature in cells.

  • What defines acids and bases?

    Acids dissociate to release H⁺ ions; bases dissociate to release OH⁻ ions or reduce H⁺ concentration.

  • What is pH?

    pH measures H⁺ ion concentration: below 7 acidic, 7 neutral, above 7 basic/alkaline.

  • What are buffers?

    Buffers maintain stable pH, essential for cellular chemical reactions.

  • What are organic compounds?

    Organic compounds contain carbon and hydrogen, mostly held by covalent bonds.

  • Why is carbon important biologically?

    Carbon can form up to four bonds, allowing formation of chains and complex structures in biological molecules.

  • What are functional groups?

    Functional groups are specific atom groups attached to organic molecules that determine chemical properties.

  • Define macromolecules and their monomers.

    Macromolecules are large molecules made of monomers: carbohydrates, lipids, proteins, nucleic acids.

  • What is dehydration synthesis?

    Dehydration synthesis joins monomers into polymers by removing water.

  • What is hydrolysis?

    Hydrolysis breaks polymers into smaller molecules by adding water.

  • Characteristics of carbohydrates?

    Carbohydrates contain C, H, O with H:O ratio ~2:1; important for energy, storage, and structure.

  • What are monosaccharides?

    Monosaccharides are simple sugars with 3–7 carbons, e.g., glucose and fructose.

  • Difference between saturated and unsaturated lipids?

    Saturated lipids have no C=C double bonds; unsaturated lipids have one or more double bonds.

  • What are phospholipids?

    Phospholipids contain glycerol, two fatty acids, and a phosphate group; major plasma membrane components.

  • What are amino acids?

    Amino acids are protein building blocks containing C, H, O, N, and sometimes S; 20 common types exist.

  • Describe protein structure levels.

    Primary: amino acid sequence; Secondary: helices and sheets; Tertiary: 3D shape; Quaternary: multiple polypeptides.

  • What are nucleotides?

    Nucleotides consist of a pentose sugar, phosphate group, and nitrogenous base; building blocks of nucleic acids.

  • Differences between DNA and RNA?

    DNA has deoxyribose, thymine, usually double-stranded; RNA has ribose, uracil, usually single-stranded.

  • What is ATP?

    ATP is the cell's main energy carrier, composed of adenosine and three phosphate groups.

  • How does ATP release energy?

    Hydrolysis of ATP's terminal phosphate releases energy, producing ADP and inorganic phosphate.