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Microbiology Unit 1: Overview and History

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  • What is Microbiology?

    Microbiology is the study of living organisms too small to be seen with the naked eye, including microbes and microorganisms.

  • Define Pathogen

    A pathogen is a disease-causing microorganism.

  • What is a Pure Culture?

    A pure culture contains a single kind of microorganism.

  • Explain Spontaneous Generation

    Spontaneous generation or abiogenesis is the disproven hypothesis that living organisms can originate from non-living matter.

  • What is Sterilization?

    Sterilization is the process of destroying all living microorganisms contaminating media.

  • Describe Pasteurization

    Pasteurization is heating foods to eliminate harmful microbes while retaining non-harmful ones.

  • Who is Robert Hooke and his contribution?

    Robert Hooke (1635-1703) made the first compound microscope in 1665 and coined the term cell from observing cork samples.

  • Who is Antoni van Leeuwenhoek?

    Antoni van Leeuwenhoek (1632-1723) created a single-lens microscope and discovered microorganisms in 1684, known as the father of microbiology.

  • What did Francesco Redi's experiment demonstrate?

    Francesco Redi disproved spontaneous generation by showing that maggots on meat came from flies, not from the meat itself.

  • Describe Louis Pasteur's contribution to microbiology

    Louis Pasteur showed microbes in air cause contamination, developed the Pasteur flask, and created vaccines for rabies, anthrax, and fowl cholera.

  • What is the Germ Theory of Disease?

    The Germ Theory states that microorganisms cause disease.

  • What are Koch's Postulates?

    Koch's postulates are criteria to prove a microorganism causes a disease, including isolation, pure culture growth, disease reproduction, and re-isolation.

  • What is Aseptic Technique?

    Aseptic techniques prevent contamination by unwanted microorganisms during handling and culturing.

  • Who was Joseph Lister and his contribution?

    Joseph Lister applied germ theory to surgery by using antiseptics to kill microbes and prevent infections.

  • What role do microbes play in photosynthesis?

    Microbes like algae and cyanobacteria perform photosynthesis, contributing about 50% of Earth's atmospheric oxygen.

  • Explain the environmental importance of microbes

    Microbes decompose dead matter, cycle nutrients, fix nitrogen, and produce greenhouse gases like CO2 and CH4.

  • What is Biotechnology in microbiology?

    Biotechnology uses microbes or their products commercially, including food preservation, pharmaceuticals, and genetic engineering.

  • Define Genetic Engineering

    Genetic engineering manipulates microbial, plant, or animal genetics to create new products or organisms.

  • Give examples of genetic engineering involving microbes

    Examples include bacteria producing insulin, yeast synthesizing hormones, and plants with delayed ripening.

  • How do microbes impact infectious diseases?

    Microbes cause diseases like malaria, AIDS, and emerging infections such as SARS and Ebola.

  • What is Bioremediation?

    Bioremediation uses microbes to degrade pollutants, industrial wastes, and household garbage.