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Ch. 2 - Chemical Principles
Tortora - Microbiology: An Introduction 14th Edition
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398Non è quello che usi tu?Cambia libro di testo
Capitolo 2, Problema 2

Radioisotopes are frequently used to label molecules in a cell. The fate of atoms and molecules in a cell can then be followed. This process is the basis for the following question.


If Pseudomonas bacteria are supplied with radioactively labeled cytosine, after a 24-hour incubation period this cytosine would most likely be found in the cells’
a. Carbohydrates.
b. DNA.
c. Lipids.
d. Water.
e. Proteins.

Guida verificata passo dopo passo
1
Step 1: Understand the role of cytosine in cellular molecules. Cytosine is one of the four nitrogenous bases found in nucleic acids, specifically DNA and RNA.
Step 2: Recall that cytosine is not a component of carbohydrates, lipids, water, or proteins. These macromolecules have different building blocks: carbohydrates are made of sugars, lipids of fatty acids and glycerol, proteins of amino acids, and water is a simple molecule (H2O).
Step 3: Since cytosine is a nitrogenous base, it is incorporated into nucleic acids. In bacteria like Pseudomonas, DNA contains cytosine as part of its structure.
Step 4: When bacteria are supplied with radioactively labeled cytosine, the label will be incorporated into newly synthesized DNA molecules during replication or repair processes.
Step 5: Therefore, after incubation, the radioactive cytosine will most likely be found in the cells' DNA.

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Role of Cytosine in Cellular Molecules

Cytosine is one of the four nitrogenous bases found in nucleic acids, specifically DNA and RNA. It pairs with guanine in DNA, making it a fundamental component of genetic material rather than carbohydrates, lipids, or proteins.
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Radioisotope Labeling in Microbiology

Radioisotope labeling involves incorporating radioactive atoms into molecules to trace their path and fate within cells. This technique helps identify where specific molecules, like cytosine, are utilized or incorporated over time.
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Macromolecular Composition of Cells

Cells are composed of major macromolecules: nucleic acids, proteins, lipids, and carbohydrates. Each has distinct building blocks; nucleic acids contain nitrogenous bases like cytosine, while proteins, lipids, and carbohydrates do not incorporate cytosine.
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Microbiome Composition