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In oxidative phosphorylation, what is the primary function of ATP synthase?
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Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation
Oxidative Phosphorylation 3
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In eukaryotic cells, where is ATP synthase (Complex V) located during oxidative phosphorylation?
A
Outer mitochondrial membrane, facing the cytosol
B
Inner mitochondrial membrane, with the catalytic head facing the matrix
C
Inner mitochondrial membrane, with the catalytic head facing the intermembrane space
D
Mitochondrial matrix, free in solution
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Recall the structure of mitochondria, which includes the outer membrane, intermembrane space, inner membrane, and matrix.
Understand that oxidative phosphorylation occurs across the inner mitochondrial membrane, where the electron transport chain complexes are embedded.
Identify that ATP synthase (Complex V) is an enzyme complex responsible for synthesizing ATP using the proton gradient established by the electron transport chain.
Recognize that ATP synthase spans the inner mitochondrial membrane, with its catalytic F1 head oriented towards the mitochondrial matrix where ATP synthesis occurs.
Conclude that ATP synthase is located in the inner mitochondrial membrane, with the F1 catalytic head facing the matrix, not the intermembrane space or free in the matrix.
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