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In glycolysis, ATP molecules are produced directly by which process?
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Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway
Glycolysis 2
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In the glycolytic pathway (conversion of one molecule of glucose to two molecules of pyruvate), how many molecules of NADH are produced per glucose under aerobic conditions?
A
1 NADH
B
0 NADH
C
4 NADH
D
2 NADH
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Recall that glycolysis is the metabolic pathway that converts one molecule of glucose into two molecules of pyruvate.
Identify the specific step in glycolysis where NADH is produced: this occurs during the oxidation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, catalyzed by glyceraldehyde-3-phosphate dehydrogenase.
Note that for each molecule of glucose, glycolysis produces two molecules of glyceraldehyde-3-phosphate, so the NADH-producing step happens twice per glucose molecule.
Calculate the total NADH molecules produced by multiplying the number of NADH molecules produced per glyceraldehyde-3-phosphate oxidation (which is 1) by 2 (because there are two glyceraldehyde-3-phosphate molecules per glucose).
Conclude that the total NADH produced per glucose molecule during glycolysis under aerobic conditions is 2 NADH molecules.
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