Biochemistry
Which amino acid in the catalytic triad acts as a nucleophile in chymotrypsin's active site?
What is the consequence of histidine 57 acting as an acid during the removal of the leaving group in the acylation phase?
What is the acyl enzyme in chymotrypsin's catalytic mechanism?
What is the result of the nucleophilic attack by serine 195 during the acylation phase?
In a scenario where the oxyanion hole is mutated, what would be the likely effect on chymotrypsin's catalytic efficiency?
What is the significance of the tetrahedral intermediate collapse during the acylation phase?
What is the final step in the deacylation phase?
