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What is the role of DNA polymerase in the process of DNA replication?
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14. DNA Synthesis
Introduction to DNA Replication
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Which of the following enzymes creates a primer for DNA polymerase during DNA replication?
A
Helicase
B
Topoisomerase
C
Ligase
D
Primase
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Understand the role of each enzyme listed in the options: Helicase unwinds the DNA double helix, Topoisomerase relieves the tension created by unwinding, and Ligase joins Okazaki fragments on the lagging strand.
Recognize that DNA polymerase requires a primer to initiate DNA synthesis. This primer is a short RNA sequence.
Identify the enzyme responsible for synthesizing the RNA primer. This enzyme is known as Primase.
Primase synthesizes a short RNA primer complementary to the DNA template strand, providing a starting point for DNA polymerase to begin adding DNA nucleotides.
Conclude that Primase is the enzyme that creates the primer necessary for DNA polymerase to function during DNA replication.
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