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How are DNA replication and PCR (Polymerase Chain Reaction) related?
A
Both processes require the enzyme helicase to unwind the DNA.
B
Both processes occur naturally within living cells.
C
Both processes result in the production of RNA molecules.
D
Both processes involve the synthesis of new DNA strands using a template.
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Understand that DNA replication and PCR are both processes that involve the synthesis of new DNA strands using a template strand of DNA.
In DNA replication, which occurs naturally in living cells, the enzyme helicase unwinds the DNA double helix to allow the replication machinery to access the template strands.
In PCR, a laboratory technique, the DNA is denatured by heat to separate the strands, eliminating the need for helicase.
Both processes use DNA polymerase enzymes to synthesize new DNA strands by adding nucleotides complementary to the template strand.
Recognize that while DNA replication occurs in cells and results in the duplication of the entire genome, PCR is used to amplify specific DNA segments in vitro, outside of living organisms.