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What is the function of DNA ligase in the gene cloning process?
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18. Biotechnology
Introduction to DNA Cloning
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What is recombinant DNA made of?
A
Restriction enzymes and target DNA.
B
Target DNA and DNA ligase.
C
DNA from two different sources.
D
A bacterial chromosome and a bacterial plasmid.
E
None are correct.
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Understand that recombinant DNA refers to a molecule formed by combining DNA from two different sources, which is a fundamental concept in genetic engineering.
Recognize that the process involves cutting DNA from different sources using restriction enzymes, which act like molecular scissors to create compatible ends.
Know that DNA ligase is the enzyme responsible for joining or 'ligating' these DNA fragments together, forming a continuous DNA strand.
Identify that the key characteristic of recombinant DNA is that it contains genetic material from two distinct origins, such as a gene from one organism inserted into the DNA of another.
Conclude that recombinant DNA is made of DNA from two different sources, which distinguishes it from DNA that comes from a single source or just the enzymes involved.
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