IndietroLab Practical 2
Termini in questo insieme (15)
What is a GMO?
Genetically Modified Organism. A plant, animal, or microorganism whose DNA has been altered using biotechnology to give it a desired trait.
What is the first step in creating a genetically modified crop?
Identify the desired trait and gene for the crop.
Why did you add glycerol to the samples you placed in wells?
To make samples denser than the electrophoresis buffer, so they would sink into the wells instead of floating around or mixing into the buffer.
What is the process of placing molecules into a porous matrix, such as a gel, and applying an electrical current to move them through the matrix?
Electrophoresis.
What does GFP stand for?
Green Fluorescent Protein.
What is the equation to calculate Rf?
Rf = Protein Marker Band Migration Distance/Tracking Dye Migration Distance
Chromatography means "color writing" and was originally used to separate plant pigments in which century?
20th Century.
There were no bands in the chromatogram, but the solvent at the bottom of the chromatography chamber has taken on a very bright color. What went wrong here?
The pigment spot was placed below the solvent level.
Identify the pigments in the graph below:
Chlorophyll a
Chlorophyll b
Carotenoids
A protein that retains its natural shape is called a?
Native Protein
What does PCR stand for?
Polymerase Chain Reaction
Molecules that are ____ _______ in the solvent system will move along _______.
more soluble, further.
The mobile phase is drawn up the TLC plate by _________ ______ , carrying the mixture of molecules along with it
Capillary action
What is the name of the organism GFP is extracted from?
Crystal Jellyfish
What is another name for protein markers?
Protein ladders, Molecular weight markers