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Given the DNA base sequence 5'-ATGCCGTA-3', what is the base sequence of the complementary RNA strand?
A
5'-UACGGCAU-3'
B
5'-ATGCCGTA-3'
C
3'-TACGGCAT-5'
D
3'-UACGGCAU-5'
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1
Identify the direction of the given DNA strand, which is 5' to 3'. The sequence is 5'-ATGCCGTA-3'.
Determine the complementary DNA bases by pairing adenine (A) with thymine (T), thymine (T) with adenine (A), cytosine (C) with guanine (G), and guanine (G) with cytosine (C).
Since the question asks for the complementary RNA strand, replace thymine (T) in the complementary strand with uracil (U), because RNA contains uracil instead of thymine.
Write the complementary RNA sequence in the 3' to 5' direction, which is opposite to the original DNA strand's 5' to 3' direction.
Verify the final complementary RNA sequence by checking each base pairing and the strand direction to ensure accuracy.