Scelta multipla
How does semi-conservative replication contribute to the prevention of mutations during DNA replication?
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17. Mutation, Repair, and Recombination
DNA Repair
Scelta multipla
Which enzyme creates nicks ahead of the replication fork to relieve tension from DNA unwinding during replication?
A
Helicase
B
Topoisomerase
C
Primase
D
DNA ligase
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Understand the role of each enzyme listed in the context of DNA replication.
Recall that Helicase unwinds the DNA double helix by breaking hydrogen bonds between base pairs, but does not relieve tension ahead of the fork.
Recognize that Primase synthesizes RNA primers needed to start DNA synthesis, but does not affect DNA tension.
Know that DNA ligase joins Okazaki fragments on the lagging strand but does not relieve supercoiling tension.
Identify that Topoisomerase is the enzyme that creates temporary nicks ahead of the replication fork to relieve the torsional strain caused by unwinding, preventing DNA tangling and supercoiling.
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