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Which form of genetic material initiates RNA interference (RNAi) in eukaryotic cells?
A
Single-stranded DNA (ssDNA)
B
Transfer RNA (tRNA)
C
Double-stranded RNA (dsRNA)
D
Messenger RNA (mRNA)
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1
Understand that RNA interference (RNAi) is a biological process in eukaryotic cells where RNA molecules inhibit gene expression by neutralizing targeted mRNA molecules.
Recall that RNAi is typically initiated by the presence of double-stranded RNA (dsRNA), which is recognized by the cellular machinery.
Know that dsRNA is processed by an enzyme called Dicer into small interfering RNAs (siRNAs), which guide the RNA-induced silencing complex (RISC) to complementary mRNA targets.
Recognize that single-stranded DNA (ssDNA), transfer RNA (tRNA), and messenger RNA (mRNA) do not initiate RNAi because they do not form the double-stranded structures required to trigger this pathway.
Conclude that the form of genetic material that initiates RNA interference in eukaryotic cells is double-stranded RNA (dsRNA).