Domanda del libro di testo
Restriction sites are palindromic; that is, they read the same in the 5' to 3' direction on each strand of DNA. What is the advantage of having restriction sites organized this way?
854
views
Ch. 20 - Recombinant DNA Technology
Klug12th EditionConcepts of GeneticsISBN: 9780135564776
Non è quello che usi tu?Cambia libro di testoScegli un libro di testo diverso
TOC.tableOfContents
- Ch. 1 - Introduction to Genetics17
- Ch. 2 - Mitosis and Meiosis36
- Ch. 3 - Mendelian Genetics51
- Ch. 4 - Extensions of Mendelian Genetics74
- Ch. 5 - Chromosome Mapping in Eukaryotes51
- Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages46
- Ch. 7 - Sex Determination and Sex Chromosomes33
- Ch. 8 - Chromosome Mutations: Variation in Number and Arrangement40
- Ch. 9 - Extranuclear Inheritance31
- Ch. 10 - DNA Structure and Analysis43
- Ch. 11 - DNA Replication and Recombination44
- Ch. 12 - DNA Organization in Chromosomes30
- Ch. 13 - The Genetic Code and Transcription54
- Ch. 14 - Translation and Proteins47
- Ch. 15 - Gene Mutation, DNA Repair, and Transposition41
- Ch. 16 - Regulation of Gene Expression in Bacteria29
- Ch. 17 - Transcriptional Regulation in Eukaryotes41
- Ch. 18 - Post-transcriptional Regulation in Eukaryotes33
- Ch. 19 - Epigenetics33
- Ch. 20 - Recombinant DNA Technology44
- Ch. 21 - Genomic Analysis36
- Ch. 22 - Applications of Genetic Engineering and Biotechnology36
- Ch. 23 - Developmental Genetics37
- Ch. 24 - Cancer Genetics34
- Ch. 25 - Quantitative Genetics and Multifactorial Traits54
- Ch. 26 - Population and Evolutionary Genetics45
Klug12th EditionConcepts of GeneticsISBN: 9780135564776Non è quello che usi tu?Cambia libro di testo
Capitolo 20, Problema 4
The human insulin gene contains a number of sequences that are removed in the processing of the mRNA transcript. In spite of the fact that bacterial cells cannot excise these sequences from mRNA transcripts, explain how a gene like this can be cloned into a bacterial cell and produce insulin.
Guida verificata passo dopo passo1
Understand that the human insulin gene contains introns, which are non-coding sequences removed during mRNA processing in eukaryotic cells but not in bacteria, as bacteria lack the machinery to splice out introns.
Recognize that to express the insulin gene in bacteria, the gene must be in a form that bacteria can read directly, meaning it should not contain introns.
Use the mature mRNA transcript of the insulin gene, which has already had the introns removed, as a template to synthesize complementary DNA (cDNA) using the enzyme reverse transcriptase.
Clone this cDNA, which represents the intron-free coding sequence of the insulin gene, into a bacterial plasmid vector that contains the necessary regulatory sequences for bacterial expression.
Transform the recombinant plasmid into bacterial cells, which can then transcribe and translate the cDNA to produce functional insulin protein, since the bacterial machinery can now read the intron-free sequence.
Risposta video verificata per un problema simile:
Questa soluzione video è stata consigliata dai nostri tutor come utile per risolvere questo problema.
Durata del video:
1mConcetti chiave
Ecco i concetti essenziali che devi comprendere per rispondere correttamente alla domanda.
Gene Structure: Exons and Introns
Eukaryotic genes often contain exons (coding regions) and introns (non-coding regions). During mRNA processing, introns are removed through splicing to produce a mature mRNA that can be translated into protein. Bacteria lack the machinery to remove introns, so direct expression of eukaryotic genes with introns in bacteria is problematic.
Video consigliato:
Percorso guidato
03:49
Ribosome Structure
cDNA and Reverse Transcription
Complementary DNA (cDNA) is synthesized from mature mRNA using reverse transcriptase. cDNA contains only exons, lacking introns, making it suitable for cloning into bacterial cells. Using cDNA ensures that bacteria can transcribe and translate the gene correctly to produce functional protein.
Video consigliato:
Percorso guidato
09:16Eukaryotic Transcription
Recombinant DNA Technology and Cloning in Bacteria
Recombinant DNA technology allows insertion of cDNA into bacterial plasmids, which are then introduced into bacterial cells. Bacteria can transcribe and translate the inserted cDNA to produce the desired protein, such as insulin, despite lacking splicing mechanisms. This method enables production of eukaryotic proteins in prokaryotic hosts.
Video consigliato:
Percorso guidato
07:39Genetic Cloning
Pratica correlata
Domanda del libro di testo
Using DNA sequencing on a cloned DNA segment, you recover the nucleotide sequence shown below. Does this segment contain a palindromic recognition sequence for a restriction enzyme? If so, what is the double-stranded sequence of the palindrome, and what enzyme would cut at this sequence?
CAGTATGGATCCCAT
703
views
Domanda del libro di testo
How can gene knockouts, transgenic animals, and gene editing techniques be used to explore gene function?
466
views
Domanda del libro di testo
What roles do restriction enzymes, vectors, and host cells play in recombinant DNA studies? What role does DNA ligase perform in a DNA cloning experiment? How does the action of DNA ligase differ from the function of restriction enzymes?
845
views
Domanda del libro di testo
Write a short essay or sketch a diagram that provides an overview of how recombinant DNA techniques help geneticists study genes.
528
views
Domanda del libro di testo
Although many cloning applications involve introducing recombinant DNA into bacterial host cells, many other cell types are also used as hosts for recombinant DNA. Why?
608
views